Goat anti-Il12b / Il12p40 (mouse) Antibody

SKU: orb19735

Description

Goat polyclonal antibody to IL12 p40

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:16000, WB: 0.3-1 μg/ml
Reactivity	Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 37kDa band observed in Mouse Spleen and Rat Liver lysates (calculated MW of 38.2kDa according to Mouse NP_032378.1 and 38.4kDa according to Rat NP_072133.1). Recommended concentration: 1-3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Il12b / Il12p40 (mouse)
Molecular Weight	38.2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti-CLMF antibody, anti-CLMF p40 antibody, anti-CLMF2 antibody, anti-IL 12 subunit p40 antibody, anti-IL 12B antibody, anti-IL-12 subunit p40 antibody, anti-IL-12B antibody, anti-IL12 subunit p40 antibody, anti-IL12B antibody, anti-interleukin 12 beta chain antibody, anti-Interleukin 12 p40 antibody, anti-Interleukin 12 subunit beta antibody, anti-Interleukin 12B antibody, anti-Interleukin-12 subunit beta antibody, anti-NK cell stimulatory factor chain 2 antibody, anti-NKSF antibody, anti-NKSF2 antibody, anti-p40 antibody, anti-IL12 p40 antiody, anti-IL 12 p40 antiody, anti-IL 12p40 antiody

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Goat anti-Il12b / Il12p40 (mouse) Antibody

orb19735 (1ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Mouse (Lane 1) and Rat (Lane 2) Skin lysate using IL12 p40 antibody

Quick Database Links

Gene Symbol

Il12b / Il12p40 (mouse)

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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