NCE2/UBE2F Antibody

SKU: orb20213

Description

Goat polyclonal antibody to UBE2F

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.05-0.2 μg/ml, IHC-P: 2.5-3.8µg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 20kDa band observed in Mouse Liver lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and to Mouse NP_080730.1). Recommended concentration: 0.05-0.2 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	NCE2 / UBE2F
Molecular Weight	21.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti UBE2F antibody, anti ubiquitin-conjugating enzyme E2F (putative) antibody, anti MGC18120 antibody, anti NCE2 antibody, anti NEDD8 conjugating enzyme antibody, anti NEDD8-conjugating enzyme antibody

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Western blot analysis of Mouse Liver lysate using UBE2F antibody

Immunohistochemical staining of Human Placenta using UBE2F antibody

Quick Database Links

Gene Symbol

NCE2 / UBE2F

NCBI Gene ID

140739

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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