Goat anti-PCSK9 (C Terminus) Antibody

SKU: orb18975

Description

Goat polyclonal antibody to PCSK9

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:16000, WB: 0.3-1 μg/ml
Reactivity	Human
Application Notes	WB: Approx 75kDa band observed in Human Adipose lysates (calculated MW of 74.3kDa according to NP_777596.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1;. IHC: Paraffin embedded Human Liver. Recommended concentration: 5-6µg/ml.

Key Properties

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Host	Goat
Target	PCSK9 (C Terminus)
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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PCSK9; FH3; NARC1; HCHOLA3; proprotein convertase subtilisin/kexin type 9; neural apoptosis regulated convertase 1; hypercholesterolemia, autosomal dominant 3; proprotein convertase subtilisin\/kexin type 9; LDLCQ1; NARC-1

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orb18975 staining (0.2ug/ml) of McA cell lysates: untransfected (1), transfected with wild type human pcsk9 (2), transfected with S127R human pcsk9 (3). Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London.

orb18975 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18975 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. This data is from a previous batch.

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Gene Symbol

PCSK9 (C Terminus)

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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