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PRODH Antibody

SKU: orb19216

Description

Goat polyclonal antibody to PRODH

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, IHC
Dilution RangeELISA: 1:16000, IHC-P: 3.75 μg/ml
ReactivityHuman
Predicted ReactivityMouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 85kDa and 25kDa in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 68.0kDa according to NP_057419.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetPRODH
Protein SequenceERDGSGTNKRDKQYQ
Molecular Weight68
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti PRODH antibody, anti proline dehydrogenase (oxidase) 1 antibody, anti HSPOX2 antibody, anti PIG6 antibody, anti PRODH1 antibody, anti PRODH2 antibody, anti SCZD4 antibody, anti TP53I6 antibody, anti FLJ33744 antibody, anti MGC148078 antibody, anti MGC148079 antibody, anti Proline oxidase, mitochondrial antibody, anti proline dehydrogenase (proline oxidase ) antibody, anti proline oxidase 2 antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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PRODH Antibody (orb19216)

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100 μg
¥ 6,890.00
DispatchUsually dispatched within 3-5 working days
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