PRODH (aa112-134) Antibody

SKU: orb125220

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Description

Goat Polyclonal to PRODH. NP_057419.4; NP_001182155.1, PMID: 18163391 and GeneID: 5625.

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	P-ELISA:1:16000, WB:0.3-1µg/ml
Reactivity	Human
Application Notes	WB: Approx75+55kDa bands observed in Human Brain (Cerebellum and Substantia nigra) lysates (calculated MW of 68.0kDakDa according to NP_057419.4 and of 56.2kDa according to NP_001182155.1). Recommended concentration: 0.3-1µg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	PRODH (aa112-134)
Protein Sequence	EDQESIQPLLRHYR
Molecular Weight	68.0; 56.2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti PRODH antibody, anti proline dehydrogenase (oxidase) 1 antibody, anti FLJ33744 antibody, anti HSPOX2 antibody, anti MGC148078 antibody, anti MGC148079 antibody, anti PIG6 antibody, anti POX antibody, anti PRODH1 antibody, anti PRODH2 antibody, anti SCZD4 antibody, anti TP53I6 antibody, anti OTTHUMP00000196496 antibody, anti OTTHUMP00000196497 antibody, anti proline dehydrogenase, mitochondrial antibody, anti proline oxidase 2 antibody, anti proline oxidase, mitochondrial antibody

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0.3 µg/ml staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PRODH (aa112-134)

NCBI Gene ID

5625

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Find More Protocols

Zareba, Ilona et al. Functional Consequences of Intracellular Proline Levels Manipulation Affecting PRODH/POX-Dependent Pro-Apoptotic Pathways in a Novel in Vitro Cell Culture Model Cell Physiol Biochem, 43, 670-684 (2017)

PubMed: 28942439