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Goat anti-S100A8 Antibody
SKU: orb12338
Description
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| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml |
| Reactivity | Human |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | S100A8 |
| Molecular Weight | 10.8 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti CAGA antibody, anti Calgranulin-A antibody, anti CFAG antibody, anti CGLA antibody, anti Chemotactic cytokine CP-10 antibody, anti CP-10 antibody, anti Cystic fibrosis antigen antibody, anti L1Ag antibody, anti Leukocyte L1 complex light chain antibody, anti MA387 antibody, anti Migration inhibitory factor-related protein 8 antibody, anti Myeloid-related protein 8 antibody, anti Neutrophil cytosolic 7 kDa protein antibody, anti NIF antibody, anti Pro-inflammatory S100 cytokine antibody, anti Protein S100-A8 antibody, anti S100 calcium binding protein A8 (calgranulin A) antibody, anti S100 calcium binding protein A8 antibody, anti S100A8 antibody, anti S100A8/S100A9 complex, included antibody, anti Urinary stone protein band A antibody, anti MRP8 antibody, anti MRP-8 antibody, anti 60B8Ag antibody
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5 µg/mL staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic/extracellular staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Goat anti-S100A8 Antibody (orb12338)
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