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SAE1/AOS1 Antibody
SKU: orb18563
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, IF |
|---|---|
| Dilution range | ELISA: 1:8000, WB: 1 μg/ml |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | SAE1 / AOS1 |
| Molecular Weight | 38.4 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti SAE1 antibody, anti AOS1 antibody, anti SUA1 antibody, anti HSPC140 antibody, anti SUMO-1 activating enzyme subunit 1 antibody, anti sentrin/SUMO-activating protein AOS1 antibody, anti SUMO-1 activating enzyme E1 N subunit antibody, anti ubiquitin-like protein SUMO-1 activating enzyme antibody, anti SUMO1 activating enzyme subunit 1 antibody, anti FLJ3091 antibody, anti UBLE1A antibody, anti activator of SUMO1 antibody, anti ubiquitin-like 1-activating enzyme E1A antibody
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orb18563 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Protocol Information
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SAE1/AOS1 Antibody (orb18563)
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