SAE2/UBA2 Antibody

SKU: orb18565

Description

Goat polyclonal to SAE2

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IF, WB
Dilution range	ELISA: 1:16000, IHC-P: 10 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Bovine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments showed an approx. 35kDa band in Human Testis and Brain extracts at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 74KDa according to NP_005490. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	SAE2 / UBA2
Molecular Weight	74
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti UBA2 antibody, anti SAE2 antibody, anti SUMO-1 activating enzyme subunit 2 antibody

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Immunofluorescence analysis of U2OS cells using SAE2 antibody

Immunofluorescence analysis of HeLa cells using SAE2 antibody

Quick Database Links

Gene Symbol

SAE2 / UBA2

NCBI Gene ID

10054

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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