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Goat anti-USP7 Antibody
SKU: orb1523887
Description
Images & Validation
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| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Between 1 and 50 |
| Target | USP7 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−TEF1; USP7; ubiquitin-specific-processing protease 7; ubiquitin thioesterase 7; ubiquitin specific protease 7 (herpes virus-associated); deubiquitinating enzyme 7; ubiquitin carboxyl-terminal hydrolase 7; Herpesvirus-associated ubiquitin-specific protease; Herpes virus-associated ubiquitin-specific protease; HAUSP; ubiquitin specific peptidase 7 (herpes virus-associated)
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Detection of mouse USP7 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-USP7 antibody orb1523887 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human USP7 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified goat anti-USP7 antibody orb1523887 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human USP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-USP7 antibody orb1523887 used for IP at 6 µg/mg lysate.
Detection of human USP7 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-USP7 (Cat. No. orb1523887) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of mouse USP7 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-USP7 (Cat. No. orb1523887) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003461.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Goat anti-USP7 Antibody (orb1523887)
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