GPC3 Rabbit Polyclonal Antibody

SKU: orb576371

Description

Rabbit polyclonal antibody to GPC3

Images & Validation

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Item 1 of 5

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human GPC3
Target	GPC3
Molecular Weight	66 kDa
Purification	Protein A purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	1.0 mg/ml
Disclaimer	For research use only

Alternative Names

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SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2

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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. GPC3 is shown in multiple isoforms and is also cleaved to a 36 kDa mature subunit.

Sample Tissue: Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.

Positive control (+): Human lung (LU), Negative control (-): Human heart (HE), Antibody concentration: 1 ug/ml.

Rabbit Anti-GPC3 antibody, Catalog Number: orb576371, Paraffin Embedded Tissue: Human Placenta cell, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

WB Suggested Anti-GPC3 Antibody Titration: 2.5 ug/ml, ELISA Titer: 1:1562500, Positive Control: Human Placenta.

Quick Database Links

Gene Symbol

GPC3

UniProt

P51654

RefSeq (Protein):NP_004475

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_004475

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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