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Glucose 6-Phosphate Isomerase Antibody / GPI

SKU: orb639688

Description

In addition to it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsIF, IHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the Glucose 6-Phosphate Isomerase antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG2a, kappa
Clone No.CPTC-GPI-1
ImmunogenA recombinant human full-length protein was used as the immunogen for the Glucose 6-Phosphate Isomerase antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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Glucose 6-Phosphate Isomerase Antibody / GPI

IHC staining of FFPE human breast carcinoma with Glucose 6-Phosphate Isomerase antibody (clone CPTC-GPI-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Glucose 6-Phosphate Isomerase Antibody / GPI

Immunofluorescence staining of fixed human MCF-7 cells with Glucose 6-Phosphate Isomerase antibody (green, clone CPTC-GPI-1) and Reddot nuclear stain (red).

Glucose 6-Phosphate Isomerase Antibody / GPI

Western blot testing of human T-47D, PC3 and HePG2 cell lysate with Glucose 6-Phosphate Isomerase antibody. Predicted molecular weight: ~63 kDa.

Glucose 6-Phosphate Isomerase Antibody / GPI

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Glucose 6-Phosphate Isomerase antibody (clone CPTC-GPI-1). These results demonstrate the foremost specificity of the CPTC-GPI-1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Glucose 6-Phosphate Isomerase Antibody / GPI

SDS-PAGE analysis of purified, BSA-free Glucose 6-Phosphate Isomerase antibody (clone CPTC-GPI-1) as confirmation of integrity and purity.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC-P
Immunohistochemistry Paraffin
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IF
Immunofluorescence
View Protocol

Glucose 6-Phosphate Isomerase Antibody / GPI (orb639688)

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20 μg
¥ 4,810.00
100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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