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HSPA5 Antibody
SKU: orb1261886
Featured
Description
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human GRP78 / BiP (NP_005338.1). |
| Target | HSPA5 |
| Molecular Weight | Observed: 78kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78
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Western blot analysis of extracts of various cell lines, using HSPA5 antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using GRP78 / BiP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GRP78 / BiP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using GRP78 / BiP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GRP78 / BiP antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using GRP78 / BiP antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using GRP78 / BiP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using GRP78 / BiP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
HSPA5
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HSPA5 Antibody (orb1261886)
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