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GSK3A Rabbit Polyclonal Antibody
SKU: orb312257
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Porcine, Rabbit, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human GSK3A (51-150/483aa) |
| Target | GSK3A |
| Molecular Weight | 54 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−GSK3A_HUMAN; Glycogen synthase kinase-3 alpha; Glycogen synthase kinase 3 alpha; EC:2.7.11.26; Serine/threonine-protein kinase GSK3A; EC:2.7.11.1; GSK 3 alpha; GSK3alpha; GSK-3 alpha; GSK3 alpha; GSK-3alpha; GSK 3A;
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (GSK3 alpha) Polyclonal Antibody, Unconjugated (orb312257) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-GSK3 alpha (orb312257) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Sample: K562 (human) cell Lysate at 40 ug, Primary: Anti-GSK3 alpha (orb312257) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 54kD, Observed band size: 54kD.
Sample: Raji Cell (Human) Lysate at 30 ug, Primary: Anti-GSK3 alpha (orb312257) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Quick Database Links
Gene Symbol
GSK3A
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
GSK3A Rabbit Polyclonal Antibody (orb312257)
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