HDAC3 Rabbit mAb [KD Validated] Antibody

SKU: orb1951614

Description

HDAC3 is a nuclear and cytoplasmic protein that deacetylates both histone (H2A, H3, H4) and non-histone substrates (RelA, SRY, p53, MEF2, PCAF and p300/CBP). HDAC3 deacetylase activity is stimulated by interactions with the N-CoR and SMRT co-repressor proteins. Together, these three proteins form a functional complex that represses transcription associated with nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD and Pit-1.

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IHC, IP, WB
Dilution range	WB 1:1000~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:200 IP 1:30
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Immunogen	A synthesized peptide derived from human HDAC3
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer	For research use only

Alternative Names

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HD3; Histone deacetylase 3; RPD3-2; SMAP45; HDAC3;

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HDAC3 Rabbit mAb [KD Validated] Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of HDAC3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

Quick Database Links

UniProt

O15379

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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Immunoprecipitation

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