HERC6 Rabbit Polyclonal Antibody

SKU: orb157504

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Description

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human HERC6 (751-850/1022aa)
Target	HERC6
Molecular Weight	115 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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EC 6.3.2.; FLJ20637; HECT domain and RCC1 like domain containing protein 6; HECT domain and RCC1-like domain-containing protein 6; Hect domain and RLD 6; HERC 6; HERC6; HERC6_HUMAN; Potential ubiquitin ligase; Probable E3 ubiquitin protein ligase HERC6; Probable E3 ubiquitin-protein ligase HERC6.

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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (orb157504) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Placenta tissue lysates, Lane 4: Rat Testis tissue lysates, Lane 5: Human HL-60 cell lysates, Lane 6: Human A431 cell lysates, Lane 7: Human MOLT4 cell lysates, Primary: Anti-HERC6 (orb157504) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kDa, Observed band size: 135 kDa.

Quick Database Links

Gene Symbol

HERC6

UniProt

Q8IVU3

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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