HERC6 Polyclonal Antibody

SKU: orb1419625

Description

Rabbit polyclonal antibody to HERC6.

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, ICC, IF, IHC-Fr, IHC-P, WB
Reactivity	Porcine, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Disclaimer	For research use only

Alternative Names

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Probable E3 ubiquitin-protein ligase HERC6, 2.3.2.26, HECT domain and RCC1-like domain-containing protein 6, HECT-type E3 ubiquitin transferase HERC6, HERC6

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Sample: Brain (Mouse) Lysate at 40 ug. Primary: Anti-HERC6 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 115kD. Observed band size: 115kD.

Sample: Embryo (Mouse) Lysate at 40 ug. Primary: Anti-HERC6 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 115 kD. Observed band size: 115 kD.

Sample: Testis (Mouse) Lysate at 40 ug. Primary: Anti-HERC6 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 115 kD. Observed band size: 115 kD.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Quick Database Links

UniProt

Q8IVU3

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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