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Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:10000; IF: 1:50-1:200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor) |
| Target | HIF1A |
| Molecular Weight | 120 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BHLHE78, MOP1, PASD8
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Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with orb633012 (HIF1a Antibody) at dilution of 1:5000.
Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using orb633012 (HIF1a antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG (H+L).
Quick Database Links
Gene Symbol
HIF1A
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HIF1A Mouse Monoclonal Antibody (orb633012)
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