HIF1A Mouse Monoclonal Antibody

SKU: orb2960000

Description

Anti-Human HIF1A Antibody (BUCC2036) is a mouse monoclonal antibody detecting HIF1A in ELISA, FCM, IP, WB. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, FC, IP, WB
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	VHH-mFc
Clone No.	BUCC2036
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Concentration	Lot dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HIF1A, MOP1, bHLHe78, Basic-helix-loop-helix-PAS protein MOP1, HIF-1-alpha, BHLHE78, Member of PAS protein 1, Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, PAS domain-containing protein 8, HIF1-alpha, PASD8, Class E basic helix-loop-helix protein 78

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow-cytometry using anti-human HIF1A antibody. HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human HIF1A antibody monoclonal antibody (Catalog # orb2960000, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

UniProt

Q16665

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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