Histone H3 Rabbit Polyclonal Antibody

SKU: orb395138

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Description

Rabbit polyclonal antibody to Histone-H3.

Research Area

Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, WB
Dilution Range	WB: 1:500-1:5000; IHC: 1:50-1:200; IF: 1:20-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	histone cluster 2, H3a
Target	Histone-H3
Molecular Weight	15-17 kDa
Purity	≥95% as determined by SDS-PAGE
Purification	Immunogen affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o

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MCF7 cells were subjected to SDS PAGE followed by western blot with orb395138 (Histone-H3 antibody) at dilution of 1:1000.

Immunofluorescent analysis of HEK-293 cells using orb395138 (Histone-H3 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb395138 (Histone-H3 Antibody) at dilution of 1:50.

Quick Database Links

Gene Symbol

Histone-H3

UniProt

Q71DI3

UniProt Details

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No UniProt data available

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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