You have no items in your shopping cart.
HIST1H3A Antibody
SKU: orb750524
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:10000, IHC: 1:100, WB: 1:2000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3. |
| Target | HIST1H3A |
| Purity | Anti-Histone H3 is directed against the human histone 3 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human and multiple other eukaryotic (mouse, rat, chicken, dog, monkey, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly). Cross-reactivity with histone-3 from other sources have not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store H3 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 83 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody
Similar Products
−- Item 1 of 48
Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control) [orb10805]
FC, IF, IHC-Fr, IHC-P, WB
Bovine, Drosophila, Porcine, Rabbit
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
1 ml, 100 μl, 500 μl, 200 μl - Item 1 of 25
Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Rabbit Polyclonal Antibody [orb614120]
ELISA, FC, ICC, IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg - Item 1 of 20
Histone H3 (tri methyl K79) Mouse Monoclonal Antibody [orb500508]
IF, IHC-Fr, IHC-P, WB
Mouse, Rat
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
200 μg, 100 μl, 200 μl, 50 μl - Item 1 of 19
Histone H3 (di methyl K36) Mouse Monoclonal Antibody [orb500366]
IF, IHC-Fr, IHC-P
Mouse, Rat
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
200 μg, 100 μl, 50 μl, 200 μl - Item 1 of 14
Histone H3.1 Rabbit Polyclonal Antibody [orb184149]
IF, IHC-Fr, IHC-P, WB
Bovine, Equine, Porcine, Primate, Rabbit, Sheep
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 50 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Immunohistochemistry of Histone H3 antibody. Tissue: human Lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: orb750524 Histone H3 antibody at 1:100. Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT.
Western Blot of Rabbit anti-Histone 3 antibody. Lane 1: Raji lysate. Lane 2: HeLa lysate. Load: 15 ug, 25 ug respectively. Primary antibody: Histone 3 antibody at 1:2000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.4 kDa, ~15.4 kDa for Histone 3. Other band(s): none.
Western Blot of Rabbit anti-Histone H3 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348667). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Ramos Lysate. Lane 10: Raji Lsyate (p/n orb348672). Lane 11: A-172 Lysate (p/n orb348708). Lane 12: NIH/3T3 Lysate (p/n orb348714). Load: 35 µg per lane. Primary antibody: Histone H3 antibody at 1:500 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 15 kDa for Histone H3.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003520.1 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HIST1H3A Antibody (orb750524)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days