Histone H3 K4me1 Antibody

SKU: orb420384

Description

Histone H3 K4me1 antibody

Images & Validation

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Tested Applications	ChIP, IF, IHC, Multiplex Assay, WB
Dilution range	ChIP: 2-5µg/million cells, IHC: 1:500, IF: 1:500, WB: 1:500
Reactivity	C. elegans, Human
Application Notes	Anti-Histone H3 [Monomethyl Lys4] antibody is tested in Western Blot and Immunofluorescence. This antibody is useful in Immunocytochemistry, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Histone H3 [Monomethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
Purity	Anti-Histone H3 [Monomethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.72 mg/ml
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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rabbit anti-Histone H3 monomethyl Lys4 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K4me1

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Immunocytochemistry of Rabbit Anti-Histone H3 [Monomethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Monomethyl Lys4] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H3 [Monomethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [Monomethyl Lys4] is expressed in green while the nuclei and alpha-tubulin were coexpressed with DAPI (blue) and Phalloidin (red).

Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys4] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys4] Antibody. Lane 1: HeLa histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Quick Database Links

UniProt

Q71DI3

RefSeq (Protein):NP_001005464

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001005464

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ChIP

Chromatin Immunoprecipitation

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