You have no items in your shopping cart.
Histone H3 K4me1/phospho T3 Antibody
SKU: orb420381
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ChIP, DOT, IF, IHC, Multiplex Assay, WB |
|---|---|
| Dilution range | IHC: 1:100, IF: 1:100, WB: 1:1000 |
| Reactivity | C. elegans, Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Histone H3 [p Thr3, Monomethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 3 of human Histone H3.2. |
| Purity | Anti-Histone H3 [p Thr3, Monomethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 0.98 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Histone H3 pT3 monomethyl Lys4 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87782, MGC87783,H3pT3/K4me1
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Dot Blot of Rabbit Histone H3 [p Thr3, Monomethyl Lys4] Antibody. Antigen: Methylated forms of the immunizing peptide. Load: 1, 10, and 100 picomoles of sample. Primary antibody: Histone H3 [p Thr3, Monomethyl Lys4] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence Microscopy of Rabbit Anti-Histone H3 [p Thr3, Monomethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Thr3, Monomethyl Lys4] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H3 [p Thr3, Monomethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [p Thr3, Monomethyl Lys4] is expressed in green while the nuclei and alpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
Western Blot of Rabbit Anti-Histone H3 [p Thr3, Monomethyl Lys4] Antibody. Lane 1: HeLa histone prep. Lane 2. NIH 3T3 histone prep. Lane 3. C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [p Thr3, Monomethyl Lys4] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Quick Database Links
UniProt
RefSeq (Protein):NP_001005464
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001005464 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
DOT
Dot Blot
ChIP
Chromatin Immunoprecipitation
Histone H3 K4me1/phospho T3 Antibody (orb420381)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a Review