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Histone H3 K4me3/K9ac Antibody
SKU: orb420431
Description
Images & Validation
−Item 1 of 3
| Tested Applications | DOT, IF, Multiplex Assay, WB |
|---|---|
| Dilution range | IF: 1:10, WB: 1:1000 |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Histone H3 K4-Me3/K9-Ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a methylation surrounding Lysine 4 and an acetylation surrounding Lysine 9 of human Histone H3. |
| Purity | Anti-Histone H3 [Trimethyl Lys4/ac Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 K4-Me3/K9-Ac from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 0.46mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Histone H3 trimethyl Lys4 Ac Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3 K4me3K9ac
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Dot Blot of Rabbit Histone H3 K4-Me3/K9-Ac Antibody. Lane 1: K4/K9 unmodified. Lane 2: K4-Me3/K9-Ac. Lane 3: K4-Me1. Lane 4: K4-Me2. Lane 5: K4-Me3. Lane 6: K9-Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 K4-Me3/K9-Ac (RABBIT) antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.
Immunofluorescence of Anti-Histone H3 Me3 K4/Ac K9 me1: Histone H3 Trimethyl Lys4/ac Lys9 antibody was tested at 1:10 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X)
Western Blot of Histone H3 K4-Me3/K9-Ac (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 1: Histone H3 nuclear marker. Lane 2: 3T3 cell lysates. Load: 35 µg per lane. Primary antibody: Histone H3 K4-Me3/K9-Ac antibody at 1.0 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 K4-Me3/K9-Ac. Other band(s): none.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
DOT
Dot Blot
Histone H3 K4me3/K9ac Antibody (orb420431)
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