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Histone H3 (tri methyl K9) Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | ChIP, DOT, ELISA, ICC, IHC-Fr, WB |
|---|---|
| Dilution range | Optimal antibody dilution should be determined by titration, however as a guideline try up to 1:1000 for western blot. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Histone H3 peptide - tri methyl K9 |
| Reactivity | Drosophila, Hamster, Human, Porcine, Primate, Rabbit, Rat, Yeast |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 6F12-H4 |
| Immunogen | Synthetic peptide of Human Histone H3, tri methylated at K9 |
| Target | Histone H3 (tri methyl K9) |
| Purification | Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS + 0.1% sodium azide |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
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Western blot analysis of Hela cell lysate using Histone H3 Me3K9 antibody
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Histone H3 (tri methyl K9) Monoclonal Antibody (orb76652)
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