HIV-1 GP120 Protein, His Tag

SKU: orb257520

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Description

Research Area

Infectious Disease & Virology

Images & Validation

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Application Notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.8 kDa. The protein migrates as 90-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293 Cells
Target	Envelope glycoprotein gp120/gp120 (HIV)
Reactivity	Virus
Tag	C-6×His
Molecular Weight	53.8 kDa
Expression Region	Thr 36 - Arg 507
Purity	>95% as determined by SDS-PAGE
Endotoxins	Less than 1.0 EU per μg by the LAL method / rFC method.
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state.
Form/Appearance	Lyophilized
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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HIV-1 GP120 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Quick Database Links

Gene Symbol

Envelope glycoprotein gp120/gp120 (HIV)

UniProt

G4XFJ5

UniProt Details

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No UniProt data available

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Protocol Information

Find More Protocols

Rao, Vasudev R. et al. The gp120 protein is a second determinant of decreased neurovirulence of Indian HIV-1C isolates compared to southern African HIV-1C isolates PLoS One, 9, e107074 (2014)

PubMed: 25188269