HLA-DRB1 Antibody (MHC II)

SKU: orb317069

Description

HLA-DRB1 binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. [UniProt]

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b, kappa
Clone No.	LN3
Immunogen	Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Immunofluorescent staining of human Ramos cells with HLA-DRB1 antibody (clone LN3, green) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil tissue with HLA-DRB1 antibody (clone LN3). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human histiocytoma tissue with HLA-DRB1 antibody (clone LN3). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Ramos cell lysate with HLA-DRB1 antibody (clone LN3). Expected molecular weight ~30 kDa.

Western blot testing of human Ramos and spleen cell lysate with HLA-DRB1 antibody (clone LN3). Expected molecular weight ~30 kDa.

Flow cytometry staining of human Raji cells with HLA-DRB1 antibody; Red = isotype control, Blue = HLA-DRB1 antibody.

Quick Database Links

UniProt

P01911

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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