Mouse HLA-DR Monoclonal Antibody

SKU: orb1520116

Description

HLA-DR Antibody

Research Area

Cardiovascular Research, Immunology & Inflammation

Images & Validation

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Item 1 of 6

Tested Applications	FC, ICC, IHC, WB
Dilution Range	WB - 1:1000; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	LN3
Immunogen	Activated human peripheral blood mononuclear cells
Target	HLA-DR
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	50 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLA-DRA1; MHC class II antigen DRA

Quality Guarantee

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Detection of human HLA-DR by western blot. Samples: Whole cell lysate (50 µg) from KG-1, SNB-75, Raji, Ramos, and SR (10 µg) cells prepared using NETN lysis buffer. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human HLA-DR by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3]. Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human HLA-DR (shaded) in Daudi cells by flow cytometry. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-mouse IgG.

Detection of human HLA-DR by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116). Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human HLA-DR by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-HLA-DR antibody [LN3] (orb1520116) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG.

Detection of human HLA-DR (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

HLA-DR

UniProt

P01903

RefSeq (Protein):NP_061984.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_061984.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ICC

Immunocytochemistry

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