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HSP A9 rabbit pAb
SKU: orb765453
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GRP75. AA range:630-679 |
| Molecular Weight | 75 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-HSPA9 antibody, anti-GRP75 antibody, anti-HSPA9B antibody, anti-Stress-70 protein antibody, anti- mitochondrial antibody, anti-75 kDa glucose-regulated protein antibody, anti-GRP-75 antibody, anti-Heat shock 70 kDa protein 9 antibody, anti-Mortalin antibody, anti-MOT antibody, anti-Peptide-binding protein 74 antibody, anti-PBP74 antibody
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Western Blot analysis of COS7 cells using HSP A9 Polyclonal Antibody diluted at 1:2000.
Immunofluorescence analysis of COS7 cells, using GRP75 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GRP75 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, using GRP75 Antibody. The lane on the right is blocked with the synthesized peptide.
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HSP A9 rabbit pAb (orb765453)
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