HSP27 Antibody (Biotin)

SKU: orb181808

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Description

Mouse monoclonal antibody against Hsp27, conjugated to Biotin.

Images & Validation

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Tested Applications	FACS, FC, ICC, IF, IHC, IP, WB
Dilution range	WB (1:5000), ICC/IF (1:200), IHC (1:100)
Reactivity	Bovine, Canine, Guinea pig, Hamster, Human, Mouse, Rat, Sheep
Application Notes	A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1 Kappa
Clone No.	8A7
Immunogen	HSP27 peptide
Target	HSP27
Molecular Weight	27kDa
Purification	Protein G Purified
Conjugation	Biotin

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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HSPB1, HSP27, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, MKBP, DMPK-binding protein

Quality Guarantee

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Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Load: 15 μg. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP27

UniProt

P04792

NCBI Gene ID

3315

RefSeq (Protein):NP_001532.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001532.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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