Hsp27 (7E5) Mouse mAb

SKU: orb1563346

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Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IF, WB
Dilution range	WB: 1/500-1/1000 IF: 1/50-1/200
Reactivity	Human, Monkey

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	IgG1
Clone No.	7E5-1F9-D9
Immunogen	Purified recombinant human Hsp27 protein fragments expressed in E.coli.
Target	HSPB1
Molecular Weight	Calculated MW: 23 kDa; Observed MW: 27 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Concentration	2.2 mg/ml
Disclaimer	For research use only

Alternative Names

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HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

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Western blot analysis of Hsp27 in Hela, A431, COS7, Lncap and MCF-7 lysates using Hsp27 antibody.

Immunocytochemistry analysis of Hsp27 (7E5) in Hela using Hsp27 antibody.

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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