You have no items in your shopping cart.
Description
Images & Validation
−
Item 1 of 7
| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution Range | Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | G3.1 |
| Immunogen | Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Similar Products
−- Item 1 of 14
- Item 1 of 12
Hsp27 Recombinant Rabbit Monoclonal Antibody [orb1499379]
FC, ICC, IF, IHC-Fr, IHC-P, WB
Rat
Human, Mouse, Rat
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl, 25 μl - Item 1 of 8
HSP27 Rabbit Polyclonal Antibody [orb10845]
FC, IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Mouse, Porcine
Human, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl - Item 1 of 7
Hsp27/HSPB1 Rabbit Polyclonal Antibody [orb215991]
FC, ICC, IF, IHC, IHC-Fr, IP, WB
Human
Rabbit
Polyclonal
Unconjugated
100 μg - Item 1 of 7
Hsp27/HSPB1 Mouse Monoclonal Antibody [orb570310]
FC, ICC, IF, IHC, WB
Human
Mouse
Monoclonal
Unconjugated
100 μg
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
Available Sizes
Select a size below
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0
Please add an antibody product to your cart first.
Usually dispatched within 5-10 working days