Cart summary

You have no items in your shopping cart.

HSP60 Antibody

SKU: orb749575

Description

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.

Images & Validation

Tested ApplicationsIF, IHC-P, WB
Dilution rangeImmunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
ReactivityHuman
Application Notes
Optimal dilution of the HSP60 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.HSPD1/780
ImmunogenRecombinant human HSPD1 protein was used as the immunogen for the HSP60 antibody.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

Similar Products

  • Hsp60/HSPD1 Antibody (monoclonal, 6G2) [orb570314]

    FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • HSP60 Rabbit Polyclonal Antibody [orb10846]

    ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Equine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • Hsp60/HSPD1 Antibody [orb251520]

    ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • HSP60 Antibody [orb69407]

    FC,  ICC,  IHC,  WB

    Human, Monkey, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μl
  • Hsp60 Antibody [orb1925739]

    IF,  IHC-P,  WB

    Bovine

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

HSP60 Antibody

IHC: Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with HSP60 antibody (clone HSPD1/780).

HSP60 Antibody

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with HSP60 antibody (clone HSPD1/780).

HSP60 Antibody

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with HSP60 antibody (clone HSPD1/780).

HSP60 Antibody

IHC: Formalin-fixed, paraffin-embedded human pancreas stained with HSP60 antibody (clone HSPD1/780).

HSP60 Antibody

Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone HSPD1/780, green) and Reddot nuclear stain (red).

HSP60 Antibody

Immunofluorescent staining of MeOH-fixed human MCF7 cells with HSP60 antibody (clone HSPD1/780, green) and Reddot nuclear stain (red).

HSP60 Antibody

Western blot testing of human HeLa lysate with HSP60 antibody (clone HSPD1/780). Predicted molecular weight: ~60 kDa.

HSP60 Antibody

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using HSP60 antibody (clone HSPD1/780). These results demonstrate the foremost specificity of the HSPD1/780 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

UniProt Details

No UniProt data available

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol

HSP60 Antibody (orb749575)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Available Sizes

Select a size below

20 μg
¥ 5,330.00
100 μg
¥ 10,140.00
DispatchUsually dispatched within 5-10 working days
Bulk Enquiry