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HSP70/HSC70 Antibody (Biotin)

SKU: orb146786
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Description

Mouse monoclonal to Hsp70 (Biotin). Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
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Images & Validation

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Tested ApplicationsICC, IF, IHC, IP, WB
Dilution rangeWB (1:1000), IHC (1:200), ICC/IF (1:200)
ReactivityBovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Yeast
Application Notes
1 µg/ml of SMC-106 was sufficient for detection of HSP70 and HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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HostMouse
ClonalityMonoclonal
IsotypeIgG2a
Clone No.BB70
ImmunogenChicken HSP70/HSP90 complex
TargetHSP70/HSC70
Molecular Weight73kDa
PurificationProtein G Purified
ConjugationBiotin

Storage & Handling

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StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

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HSPA1A, HSPA1B, HSPA1, HSPA8, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSC70, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein

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    1. HSP70/HSC70 Antibody (Biotin)
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    HSP70/HSC70 Antibody (Biotin) [orb146752]

    FC,  ICC,  IEM,  IF,  IHC,  IP,  WB

    Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Sheep

    Mouse

    Monoclonal

    Biotin

    200 μg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

HSP70/HSC70 Antibody (Biotin)

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.

HSP70/HSC70 Antibody (Biotin)

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown.

HSP70/HSC70 Antibody (Biotin)

Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.

HSP70/HSC70 Antibody (Biotin)

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.

HSP70/HSC70 Antibody (Biotin)

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control.

HSP70/HSC70 Antibody (Biotin)

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control.

Quick Database Links

Gene Symbol
HSP70/HSC70
UniProt
P08106
NCBI Gene ID
423504
RefSeq (Protein):NP_001006686.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
ProteinNP_001006686.1

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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IF
Immunofluorescence
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ICC
Immunocytochemistry
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IP
Immunoprecipitation
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HSP70/HSC70 Antibody (Biotin) (orb146786)

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200 μg
¥ 9,360.00
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