HSP90 alpha Antibody (FITC)

SKU: orb147297

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Description

Mouse monoclonal to Hsp90 alpha antibody (FITC)

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Tested Applications	ELISA, ICC, IF, IHC, IP, WB
Dilution range	WB (1:2000), ICC/IF (1:100)
Reactivity	Human, Mouse, Rat
Application Notes	0.5 µg/ml of SMC-147 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	2G5.G3
Immunogen	Recombinant Full Length Human HSP90alpha Protein
Target	HSP90 alpha
Molecular Weight	90kDa
Purification	Protein G Purified
Conjugation	FITC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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HSP90AA1, HSP90-alpha, HSP90A, HSPCA, HSPC1, Heat shock protein HSP 90-alpha, Heat shock 90 kDa protein 1 alpha isoform, HSPCAL3, HSP86, HSP89A, HSP90Alpha

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Western Blot analysis of Rat tissue lysate showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP90 alpha

UniProt

P07900

NCBI Gene ID

3320

RefSeq (Protein):NP_001017963.2

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001017963.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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