HSP90 alpha/beta Antibody

SKU: orb1822490

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Description

Mouse Anti-Human HSP90 alpha/beta Monoclonal IgG2a

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Tested Applications	ELISA, ICC, IF, IHC, WB
Dilution range	WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Reactivity	Human, Mouse, Rat, Saccharomyces, Yeast
Application Notes	1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	Hyb-K41220A
Immunogen	Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304
Target	HSP90 alpha/beta
Molecular Weight	90kDa
Purification	Protein G Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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HSP90AA1, HSP90AB1, HSP90-alpha, HSP90-beta, HSP90A, HSP90B, HSP89A, HSP86, HSP84, HSP90Alpha, HSPCA, HSPC1, HSPC2, HSPCB, HSPCAL3, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90-beta, Heat shock 90 kDa protein 1 alpha isoform, Heat shock 84 kDa protein, D6S182, FLJ26984

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.

Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.

Quick Database Links

Gene Symbol

HSP90 alpha/beta

UniProt

P08238, P07900

NCBI Gene ID

3326, 3320

RefSeq (Protein):NP_031381.2, NP_001017963.2

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_031381.2, NP_001017963.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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