HSP90 Antibody (HRP)

SKU: orb146890

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Description

Mouse monoclonal to Hsp90 antibody (HRP)

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Tested Applications	AM, IHC, WB
Dilution range	WB (1:1000), IHC (1:2000)
Reactivity	Fungi, Gallus, Human, Insect, Invertebrate, Mouse, Plant, Rabbit, Rat
Application Notes	1 µg/ml of SMC-112 was sufficient for detection of HSP90 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	AC-16
Immunogen	Heat shock protein 90 from the water mold Achyla ambisexualis
Target	HSP90
Molecular Weight	90kDa
Purification	Protein G Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B

Quality Guarantee

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Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone AC-16. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:2000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells.

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone AC-16. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:2000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Mostly inflammatory cells, some mucosa.

Quick Database Links

Gene Symbol

HSP90

UniProt

Q8LLI5

NCBI Gene ID

4768

RefSeq:AAM90675.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAM90675.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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