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HSP90A rabbit pAb Antibody
SKU: orb765460
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP90A. AA range:683-732 |
| Molecular Weight | 85 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-HSP90AA1 antibody, anti-HSP90A antibody, anti-HSPC1 antibody, anti-HSPCA antibody, anti-Heat shock protein HSP 90-alpha antibody, anti-Heat shock 86 kDa antibody, anti-HSP 86 antibody, anti-HSP86 antibody, anti-Renal carcinoma antigen NY-REN-38 antibody
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Immunofluorescence analysis of Hela cell. 1, HSP90A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 Monoclonal Antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Western Blot analysis of various cells using HSP90A Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of HeLa cells using HSP90A Polyclonal Antibody diluted at 1:2000.
Immunofluorescence analysis of NIH/3T3 cells, using HSP90A Antibody. The picture on the right is blocked with the synthesized peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HSP90A rabbit pAb Antibody (orb765460)
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