HSP90AA1/Hsp90 Alpha A1 Antibody

SKU: orb1538151

Description

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, WB
Dilution range	ELISA (1:5000 - 1:25000), IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:2500)
Reactivity	Drosophila, Gallus, Human, Monkey, Mouse, Rat
Application Notes	Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml. Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 289-300 of human Hsp90 protein.
Target	HSP90AA1 / Hsp90 Alpha A1
Purification	Affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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HSP90AA1, EL52, HSP86, Hsp89, HSP89A, HSPC1, Heat shock 86 kDa, Hsp90, HSP90A, HSP90N, HSPCA, HSPCAL4, HSPN, LAP2, HSP 86, HSPCAL1

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Quick Database Links

Gene Symbol

HSP90AA1 / Hsp90 Alpha A1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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