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HSPA5 Antibody
SKU: orb1271043
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Description
Images & Validation
−Item 1 of 6
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5. |
| Target | HSPA5 |
| Molecular Weight | 72 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78
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Antibody is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.
Western blot analysis of anti-HSPA5 Pab in HL60 cell line lysates (35 ug/lane).
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Formalin-fixed and paraffin-embedded human Testis tissue reacted with HSPA5 antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Confocal immunofluorescent analysis of HSPA5 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
HSPA5 Antibody (orb1271043)
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