HSPBAP1 Rabbit Polyclonal Antibody

SKU: orb1559

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Description

Research Area

Jumonji Containing Proteins, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human HSPBAP1 (151-250/488aa)
Target	HSPBAP1
Molecular Weight	54 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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27 KdA heat shock protein-associated protein 1; HBAP1_HUMAN; hspbap; HSPB1-associated protein 1; HSPBAP1; PASS1; Protein associated with small stress protein 1; protein associating with small stress protein.

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Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated (orb1559) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: 293T Cell (human) Lysate at 40 ug, Lymph node (mouse) Lysate at 40 ug, HepG2 Cell (human) Lysate at 40 ug, Testis (mouse) Lysate at 40 ug, Primary: Anti-HSPBAP1 (orb1559) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55 kD.

Quick Database Links

Gene Symbol

HSPBAP1

UniProt

Q96EW2

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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