Human Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) ELISA Kit

SKU: orb777822

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Alpha(SCNN1a).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCNN1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCNN1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCNN1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCNN1a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.063 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ENaCa ELISA Kit, ENaCalpha ELISA Kit, SCNEA ELISA Kit, SCNN1 ELISA Kit, Sodium Channel,Nonvoltage-Gated 1,Alpha ELISA Kit, Epithelial Na(+) channel subunit alpha ELISA Kit, Nonvoltage-gated sodium channel 1 subunit alpha ELISA Kit

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Human Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) ELISA Kit

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UniProt

P37088

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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