Human Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g) ELISA Kit

SKU: orb778103

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Gamma(SCNN1g).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCNN1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCNN1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCNN1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCNN1g in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.118 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ENaCg ELISA Kit, ENaCgamma ELISA Kit, PHA1 ELISA Kit, SCNEG ELISA Kit, Sodium Channel,Nonvoltage-Gated 1,Alpha ELISA Kit, Epithelial Na(+) channel subunit gamma ELISA Kit, Nonvoltage-gated sodium channel 1 subunit gamma ELISA Kit

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Human Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g) ELISA Kit

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UniProt

P51170

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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