Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

SKU: orb778399

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 11A(Bcl11A).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Bcl11A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Bcl11A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Bcl11A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Bcl11A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.118 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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BCL11A-L ELISA Kit, BCL11A-S ELISA Kit, BCL11A-XL ELISA Kit, CTIP1 ELISA Kit, EVI9 ELISA Kit, Ecotropic Viral Integration Site 9 ELISA Kit, COUP-TF-interacting protein 1 ELISA Kit, Ecotropic viral integration site 9 homolog ELISA Kit, Zinc finger protein 856 ELISA Kit

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Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

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UniProt

Q9H165

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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