Human BAX protein

SKU: orb391282

Description

Recombinant human BAX protein is produced by our E. coli expression system and the target gene encoding Met1-Gln171 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus.

Research Area

Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Target	BAX
Reactivity	Human
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Protein Sequence	MGSSHHHHHH SSGLVPRGSH MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG LLSYFGTPTW QLEHHHHHH

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4

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Quick Database Links

Gene Symbol

BAX

UniProt

Q07812

NCBI Gene ID

581

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Datasheet

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Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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