Human BTLA Protein, mFc-His Tag

SKU: orb689405

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Description

Recombinant human BTLA protein with C-terminal mouse Fc and 6×His tag

Research Area

Epigenetics, Immunology

Images & Validation

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Item 1 of 4

Key Properties

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Source	Mammalian
Expression System	HEK293
Target	BTLA
Reactivity	Virus
Tag	C-Mouse Fc and 6×His Tag
Molecular Weight	The protein has a predicted molecular mass of 41.3 kDa after removal of the signal peptide.
Expression Region	BTLA(Lys31-Ser150) mFc(Pro99-Lys330) 6×His tag
Purification	Purified from cell culture supernatant by affinity chromatography
Purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Form/Appearance	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Buffer/Preservatives	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BTLA;CD272

Quality Guarantee

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SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His tagged protein (orb689446) can bind Anti-SARS-CoV-2 S protein RBD Neutralizing Antibody (A neutralizing monoclonal antibody clone currently under clinical investigation from collaboration company) in a linear range of 0.24-9.141 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) S-RBD, mFc-His tagged protein (orb689446) can bind Anti-SARS-CoV-2 Neutralizing antibody CB6 orb689316 in a linear range of 0.24-15.62 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) S-RBD, mFc-His tagged protein (orb689446) can bind Human ACE2, hFc Tagged protein orb689421 in a linear range of 0.488-49.83 ng/ml.

Quick Database Links

Gene Symbol

BTLA

UniProt

Q7Z6A9

UniProt Details

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No UniProt data available

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