Human CD3 epsilon Protein, His Tag

SKU: orb257326

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Description

Human CD3 epsilon, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).

Research Area

Disease Biomarkers

Images & Validation

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Application Notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. Maybe caused by unknown PTMs, the protein migrates as 18-19 kDa and 21-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and 35-43 kDa under non-reducing (NR) condition (SDS-PAGE).

Key Properties

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Expression System	HEK293
Target	CD3 epsilon
Reactivity	Human
Tag	C-10×His
Molecular Weight	16.9 kDa
Expression Region	Asp 23 - Asp 126
Purity	90%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 24 months in lyophilized state;. -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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SDS-PAGE analysis of Human CD3 epsilon protein

Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 ug/mL (100 uL/well) can bind Human CD3E, His Tag with a linear range of 0.4-6.25 ng/mL.

Quick Database Links

Gene Symbol

CD3 epsilon

UniProt

P07766

UniProt Details

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No UniProt data available

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