Human CD30 Mouse Monoclonal Antibody

Blank control (black line): SR. Primary Antibody (green line): Mouse Anti-human CD30 antibody (orb1172664), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.