CD3E Rabbit Monoclonal Antibody

SKU: orb2975076

Description

Anti-Human CD3E Antibody (BWEJVX3) is a rabbit monoclonal antibody detecting T3E in FCM. Suitable for Human. Highlights Rabbit Monoclonal: High specificity, affinity, and excellent lot-to-lot consistency. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	FC
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	IgG, kappa
Clone No.	BWEJVX3
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Concentration	Lot dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain, UCHT1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow-cytometry using anti-human CD3E antibody. CD3E Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # orb2975076, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD3E antibody. Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # orb2975076, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD3E antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # orb2975076, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

UniProt

P07766

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No UniProt data available

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Protocol Information

Flow Cytometry

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