Human CD86 protein

SKU: orb391431

Description

Recombinant human CD86 protein is produced by our Mammalian expression system and the target gene encoding Ala24-Pro247 is expressed with a 6His tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Target	CD86
Reactivity	Human
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity	Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Protein Sequence	APLKIQAYFN ETADLPCQFA NSQNQSLSEL VVFWQDQENL VLNEVYLGKE KFDSVHSKYM GRTSFDSDSW TLRLHNLQIK DKGLYQCIIH HKKPTGMIRI HQMNSELSVL ANFSQPEIVP ISNITENVYI NLTCSSIHGY PEPKKMSVLL RTKNSTIEYD GIMQKSQDNV TELYDVSISL SVSFPDVTSN MTIFCILETD KTRLLSSPFS IELEDPQPPP DHIPVDVHHH HHH

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86

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Human B7-2 Protein, mFc-His Tag [orb689395]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 52.4 kDa after removal of the signal peptide.
Source:	Mammalian

Sizes Available: 10 μg, 100 μg, 50 μg

Recombinant Human B7-2 Protein [orb2995097]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 26.69 KDa. Observed: 57-66 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg
Recombinant Human B7-2 Protein (Biotin) [orb2993754]
Conjugation:
Biotin
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 28.4 KDa. Observed: 55-80 KDa, reducing conditions
Sizes Available: 20 μg, 100 μg
Recombinant Cynomolgus B7-2 Protein [orb2994271]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 52.5 KDa. Observed: 70-100 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg
Recombinant Mouse B7-2 Protein [orb2994404]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 52.2 KDa. Observed: 68-95 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg

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Quick Database Links

Gene Symbol

CD86

UniProt

P42081

NCBI Gene ID

942

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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