Human Colony Stimulating Factor Receptor, Macrophage (MCSFR) ELISA Kit

SKU: orb779047

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor Receptor, Macrophage(MCSFR).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCSFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCSFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MCSFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCSFR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.126 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD115 ELISA Kit, MCFSR ELISA Kit, M-CSFR ELISA Kit, CSF1R ELISA Kit, CSF1-R ELISA Kit, C-FMS ELISA Kit, CSFR ELISA Kit, FIM2 ELISA Kit, FMS ELISA Kit, Colony Stimulating Factor 1 Receptor ELISA Kit, Formerly McDonough Feline Sarcoma Viral(v-Fms)oncogene Homolog ELISA Kit

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Human Colony Stimulating Factor Receptor, Macrophage (MCSFR) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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